Part:BBa_K5068012

RppA

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Profile

Name：RppA

Origin：S. griseus

Length: 1158bp

Usage and Biology

In order to visualize the more efficient expression of genes, we also selected a reporter gene 1,3,6,8-tetrahydroxynaphthalene synthase (RPPA). Reported studies have reutilized type III polyketide synthase (PKS) 1,3,6,8-tetrahydroxynaphthalene synthase (THNS); RppA (also known as RppA) developed a simple and powerful enzyme-coupled malonyl-CoA biosensor. RppA is responsible for the conversion of malonyl-CoA to 1,3,6,8-tetrahydroxynaphthalene (THN) and then to flavomycin (Fig 1). Because flavin is red, it can be used as a direct colorimetric indicator of intracellular malonyl-CoA levels. Therefore, we draw on the reporter gene of the sensor[1].

Fig. 1. A type III PKS RppA can be repurposed as a malonyl-CoA biosensor.

Reference

1. Dongsoo Y, Jun W K, Min S Y, et al. Repurposing type III polyketide synthase as a malonyl-CoA biosensor for metabolic engineering in bacteria. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115(40): 9835-9844.